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Browse GrapePLEX Experiment Data

Choose an experiment:
Grape_Bud_Dormancy
Anne Fennell, South Dakota State University (anne.fennell@sdstate.edu)
Experiment design (167 hybridizations)
genotype
•Seyval (SV, Seyve Villard 5-276) •V. riparia (VR, PI588259)
year
•1 •2
photoperiod
•15 h •13 h
time
•1 days •3 days •7 days •14 days •21 days •28 days •42 days

Bud endodormancy induction response of two genotypes (‘Seyval’ a hybrid white wine grape and V. riparia, PI588259 a native north american speci...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Grape_Bud_Dormancy
Accession No: VV18
Microarray: Grape
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Seyval (SV, Seyve Villard 5-276)   •V. riparia (VR, PI588259)
year
•1   •2
photoperiod
•15 h   •13 h
time
•1 days  •3 days  •7 days  •14 days  •21 days  •28 days  •42 days
Quality Control: biological replicates
Treatment summary:
 genotype  year  photoperiod  time  # replicates
 Seyval (SV, Seyve Villard 5-276)  1  15 h  1 days  3
 Seyval (SV, Seyve Villard 5-276)  1  15 h  3 days  3
 Seyval (SV, Seyve Villard 5-276)  1  15 h  7 days  3
 Seyval (SV, Seyve Villard 5-276)  1  15 h  14 days  3
 Seyval (SV, Seyve Villard 5-276)  1  15 h  21 days  3
 Seyval (SV, Seyve Villard 5-276)  1  15 h  28 days  3
 Seyval (SV, Seyve Villard 5-276)  1  15 h  42 days  3
 Seyval (SV, Seyve Villard 5-276)  1  13 h  1 days  3
 Seyval (SV, Seyve Villard 5-276)  1  13 h  3 days  3
 Seyval (SV, Seyve Villard 5-276)  1  13 h  7 days  3
 Seyval (SV, Seyve Villard 5-276)  1  13 h  14 days  3
 Seyval (SV, Seyve Villard 5-276)  1  13 h  21 days  3
 Seyval (SV, Seyve Villard 5-276)  1  13 h  28 days  3
 Seyval (SV, Seyve Villard 5-276)  1  13 h  42 days  3
 Seyval (SV, Seyve Villard 5-276)  2  15 h  1 days  2
 Seyval (SV, Seyve Villard 5-276)  2  15 h  3 days  3
 Seyval (SV, Seyve Villard 5-276)  2  15 h  7 days  3
 Seyval (SV, Seyve Villard 5-276)  2  15 h  14 days  3
 Seyval (SV, Seyve Villard 5-276)  2  15 h  21 days  3
 Seyval (SV, Seyve Villard 5-276)  2  15 h  28 days  3
 Seyval (SV, Seyve Villard 5-276)  2  15 h  42 days  3
 Seyval (SV, Seyve Villard 5-276)  2  13 h  1 days  3
 Seyval (SV, Seyve Villard 5-276)  2  13 h  3 days  3
 Seyval (SV, Seyve Villard 5-276)  2  13 h  7 days  3
 Seyval (SV, Seyve Villard 5-276)  2  13 h  14 days  3
 Seyval (SV, Seyve Villard 5-276)  2  13 h  21 days  3
 Seyval (SV, Seyve Villard 5-276)  2  13 h  28 days  3
 Seyval (SV, Seyve Villard 5-276)  2  13 h  42 days  3
 V. riparia (VR, PI588259)  1  15 h  1 days  3
 V. riparia (VR, PI588259)  1  15 h  3 days  3
 V. riparia (VR, PI588259)  1  15 h  7 days  3
 V. riparia (VR, PI588259)  1  15 h  14 days  3
 V. riparia (VR, PI588259)  1  15 h  21 days  3
 V. riparia (VR, PI588259)  1  15 h  28 days  3
 V. riparia (VR, PI588259)  1  15 h  42 days  3
 V. riparia (VR, PI588259)  1  13 h  1 days  3
 V. riparia (VR, PI588259)  1  13 h  3 days  3
 V. riparia (VR, PI588259)  1  13 h  7 days  3
 V. riparia (VR, PI588259)  1  13 h  14 days  3
 V. riparia (VR, PI588259)  1  13 h  21 days  3
 V. riparia (VR, PI588259)  1  13 h  28 days  3
 V. riparia (VR, PI588259)  1  13 h  42 days  3
 V. riparia (VR, PI588259)  2  15 h  1 days  3
 V. riparia (VR, PI588259)  2  15 h  3 days  3
 V. riparia (VR, PI588259)  2  15 h  7 days  3
 V. riparia (VR, PI588259)  2  15 h  14 days  3
 V. riparia (VR, PI588259)  2  15 h  21 days  3
 V. riparia (VR, PI588259)  2  15 h  28 days  3
 V. riparia (VR, PI588259)  2  15 h  42 days  3
 V. riparia (VR, PI588259)  2  13 h  1 days  3
 V. riparia (VR, PI588259)  2  13 h  3 days  3
 V. riparia (VR, PI588259)  2  13 h  7 days  3
 V. riparia (VR, PI588259)  2  13 h  14 days  3
 V. riparia (VR, PI588259)  2  13 h  21 days  3
 V. riparia (VR, PI588259)  2  13 h  28 days  3
 V. riparia (VR, PI588259)  2  13 h  42 days  3
Total hybridizations: 167
Description: Bud endodormancy induction response of two genotypes (‘Seyval’ a hybrid white wine grape and V. riparia, PI588259 a native north american species) was compared under long and short photoperiod. Three separate replicates (5 plants/replicate) were treated in each of 2 separate years (2007 and 2008) to generate paradormant (LD) and same aged endodormancy-induced (SD) buds for transcriptomic, proteomic and metabolomic analysis. Potted, spur-pruned two to six-year-old vines were removed from cold storage (Seyval 3-19-07, 3/18/08; V. riparia 3/26/07, 3/24/08) and grown under a LD (15 h) at 25/20 + 3C day/night temperatures (D/N). When vines reached 12-15 nodes they were randomized into groups for differential photoperiod treatments. On 4/30/07 and 4/28/08 LD and SD (13 h) treatments were imposed with automated photoperiod system (VRE Greenhouse Systems). Temperatures were maintained at 25/20 + 3C D/N. Three replications (5 vines/replication) were harvested between 5/07-6/07 and then again in 5/08-6/08. At 1, 3, 7, 14, 21, 28 and 42 days of differential photoperiod treatment, buds were harvested from nodes 3 to 12 (from the base of the shoot) of each separate replicate, immediately frozen in liquid nitrogen, and placed at -80C for future RNA, protein and metabolite extraction. These time points encompass early reversible phases as well as key time points during transition to irreversible endodormancy development. After photoperiod treatments and bud harvests, all pruned vines were returned to LD and monitored for bud endodormancy. The endodormant vines were identified after 28 days and moved to cold storage. The nondormant vines were allowed to grow again and induced into dormancy at a later date.
Acknowledgement:This study was funded by NSF Grant DBI0604755 and funds from the South Dakota Agriculture Experiment Station.

Submitted at NCBI GEO via PLEXdb.

Publication: none
Created: 2009-11-17 11:22:38
Last Update: 2010-03-09 09:05:27
GEO Accession GSE66913
Submitter: Anne Y Fennell
Name: Anne Fennell
Institution: South Dakota State University
Head of Laboratory: Anne Fennell
email(s):
Homepage:

 
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