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Browse GrapePLEX Experiment Data

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Expression data from 35S::VvCBF4-overexpressing grapevines
Richard Leonard Tillett, University of Nevada Reno, Department of Biochemistry & Molecular Biology, Reno, Nevada (rltillett@unr.edu)
Experiment design (8 hybridizations)
genetic line
•VvCBF4 overexpressor •control

This experiment has been imported by PLEXdb from NCBI GEO (GSE29948)

Series_summary:
Overexpression of a grapevine C-repeat binding f...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Expression data from 35S::VvCBF4-overexpressing grapevines
Accession No: VV31
Microarray: Grape
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•VvCBF4 overexpressor   •control
Quality Control: biological replicates
Treatment summary:
 genetic line  # replicates
 VvCBF4 overexpressor  4
 control  4
Total hybridizations: 8
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE29948)

Series_summary:
Overexpression of a grapevine C-repeat binding factor (CBF) gene, VvCBF4 in cv. “Freedom” was found to improve freezing survival in non-cold-acclimated vines. To understand the mechanistic basis of VvCBF4 transgene action, a transgenic line (‘9-12’) was genotyped using microarray-based mRNA expression profiling.

Series_overall_design:
Young grapevines (cv. 'Freedom') were grown in soil for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare vines transformed with the VvCBF4 transcription factor, expressed under the 35S CaMV promoter (line '9-12') vs. an empty vector transformant (line '8-6'). We harvested the whole aerial tissues of four biological replicates, each.
Publication: none
Created: 2011-09-29 11:37:45
Last Update: 2011-10-04 09:32:52
Released: 2011-10-04
GEO Accession GSE29948
Submitter: PLEXdb Curator
Name: Richard Leonard Tillett
Institution: University of Nevada Reno, Department of Biochemistry & Molecular Biology, Reno, Nevada
Head of Laboratory: John C. Cushman
email(s):
Homepage:

 
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