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Browse MaizePLEX Experiment Data

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Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression - Embryo data
Nathan,M,Springer, University of Minnesota, Saint Paul, Minnesota (springer@umn.edu)
Experiment design (12 hybridizations)
genotype
•Mo17 •B73 •Mo17xB73 •B73xMo17

This experiment has been imported from NCBI GEO(GSE8179). Series_summary Microarray analysis of gene expression patterns in immature ear, see...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Cis-transcriptional variation in maize inbred lines B73 and Mo17 leads to additive expression - Embryo data
Accession No: ZM13
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•Mo17   •B73   •Mo17xB73   •B73xMo17
Quality Control: biological replicates
Treatment summary:
 genotype  # replicates
 Mo17  3
 B73  3
 Mo17xB73  3
 B73xMo17  3
Total hybridizations: 12
Description: This experiment has been imported from NCBI GEO(GSE8179).

Series_summary
Microarray analysis of gene expression patterns in immature ear, seedling, and embryo tissues from the maize inbred lines B73 and Mo17 identified numerous genes with variable expression. Some genes had detectable expression in only one of the two inbreds; most of these genes were detected in the genomic DNA of both inbreds, indicating that the expression differences are likely caused by differential regulation rather than by differences in gene content. Gene expression was also monitored in the reciprocal F1 hybrids B73xMo17 and Mo17xB73. The reciprocal F1 hybrid lines did not display parental effects on gene expression levels. Approximately 80% of the differentially expressed genes displayed additive expression patterns in the hybrids relative to the inbred parents. The approximately 20% of genes that display nonadditive expression patterns tend to be expressed at levels within the parental range, with minimal evidence for novel expression levels greater than the high parent or less than the low parent. Analysis of allele-specific expression patterns in the hybrid suggested that intraspecific variation in gene expression levels is largely attributable to cis-regulatory variation in maize. Collectively, our data suggest that allelic cis-regulatory variation between B73 and Mo17 dictates maintenance of inbred allelic expression levels in the F1 hybrid, resulting in additive expression patterns.

Series_overall_design
Affymetrix expression profiling was used to study gene expression in 19 day after pollination embryo tissue of maize. Three biological replicates were performed for four different genotypes; B73, Mo17, B73xMo17 and Mo17xB73.
Publication: none
Created: 2009-04-16 14:19:29
Last Update: 2009-04-16 16:15:25
GEO Accession GSE8179
Submitter: PLEXdb Curator
Name: Nathan,M,Springer
Institution: University of Minnesota, Saint Paul, Minnesota
Head of Laboratory: --
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