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Browse MaizePLEX Experiment Data

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Gibberellin-induced gene expression in maize mesocotyl under deep-sowing condition
Guoying,,Wang, Chinese Academy of Agricultural Sciences, Beijing, China (gywang@caas.net.cn)
Experiment design (6 hybridizations)
compound
•control •GA3 •UCZ

This experiment has been imported by PLEXdb from NCBI GEO (GSE15048)

Series_summary:
In order to elucidate the molecular mechanism of...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Gibberellin-induced gene expression in maize mesocotyl under deep-sowing condition
Accession No: ZM15
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
compound
•control   •GA3   •UCZ
Quality Control: biological replicates
Treatment summary:
 compound  # replicates
 control  2
 GA3  2
 UCZ  2
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE15048)

Series_summary:
In order to elucidate the molecular mechanism of gibberellin (GA)-induced mesocotyl elongation, gene expression profiling analyses were performed in a deep-sowing tolerant maize inbred line 3681-4. Gene expression studies combing Affymetrix GeneChip analysis and Real-time PCR were employed to determine the molecular mechanism underlying GA promotion of maize mesocotyl elongation. These studies showed that the GA receptor GID1, the transcriptional factor MYB, and the genes encoding DELLA protein DWRF8, kinases, Raf, LRR, RLCK, and involved in flavonoid biosynthesis, aminosugars metabolism, cell wall synthesis and modification, might play critical roles in maize mesocotyl elongation.

Series_overall_design:
In two independent experiments, we generate 10-day-old maize mesocotyl-specific gene expression profiles through comparing genome-wide expression patterns of GA3 treatment and UCZ (a GA biosynthesis inhibitor) treatment under 20 cm deep-sowing condition in darkness by using 17,555 Affymetrix maize whole genome array.
Publication: none
Created: 2009-04-21 17:45:19
Last Update: 2009-04-23 14:17:20
GEO Accession GSE15048
Submitter: PLEXdb Curator
Name: Guoying,,Wang
Institution: Chinese Academy of Agricultural Sciences, Beijing, China
Head of Laboratory: --
email(s):
Homepage:

 
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