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Browse MaizePLEX Experiment Data

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Non-additive and imprinted gene expression in hybrid maize endosperm_19DAP
Nathan,M,Springer, University of Minnesota, Saint Paul, Minnesota (springer@umn.edu)
Experiment design (12 hybridizations)
genetic line
•Mo17 •B73 •Mo17xB73 •B73xMo17

This experiment has been imported by PLEXdb from NCBI GEO (GSE8278)

Series_summary:
The nuclear content of the plant endosperm is the...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Non-additive and imprinted gene expression in hybrid maize endosperm_19DAP
Accession No: ZM17
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•Mo17   •B73   •Mo17xB73   •B73xMo17
Quality Control: biological replicates
Treatment summary:
 genetic line  # replicates
 Mo17  3
 B73  3
 Mo17xB73  3
 B73xMo17  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE8278)

Series_summary:
The nuclear content of the plant endosperm is the result of the contribution two maternal genomes and a single paternal genome. This 2:1 dosage relationship provides a unique system for studying the additivity of gene expression levels in reciprocal hybrids. A combination of microarray profiling and allele-specific expression analysis was performed using RNA isolated from endosperm tissues of maize inbred lines B73 and Mo17 and their reciprocal hybrids at two developmental stages, 13 and 19 days after pollination. By assessing the relative levels of expression in the reciprocal hybrids it was possible to determine the prevalence of additive and non-additive expression patterns. While the majority of differentially expressed genes displayed additive expression patterns in the endosperm, approximately 10% of the genes displayed non-additive expression patterns including maternal-like, paternal-like, dominant high-parent, dominant low-parent and expression patterns outside the range of the inbreds. The frequency of hybrid expression patterns outside of the parental range in maize endosperm tissue is much higher than that observed for vegetative tissues. For a set of 90 genes allele-specific expression assays were employed to monitor allelic bias and regulatory variation. Eight of these genes exhibited evidence for maternally or paternally biased expression at multiple stages of endosperm development and are potential examples of differential imprinting. Collectively, our data indicate that parental effects on gene expression are much stronger in endosperm than in vegetative tissues, and that endosperm imprinting may be far more common than previously estimated.

Keywords: genotype comparison

Series_overall_design:
Gene expression levels were profiled in 19 day after pollination endosperm tissue from four maize genotypes; B73, Mo17, Mo17xB73 and B73xMo17.
Publication: 'Nonadditive expression and parent-of-origin effects identified by microarray and allele-specific expression profiling of maize endosperm.', Stupar RM, Hermanson PJ, Springer NM.
Plant Physiol 2007 Oct;145(2):411-25.
pubmed: 17766400
Created: 2009-04-27 14:01:34
Last Update: 2009-04-27 14:22:06
GEO Accession GSE8278
Submitter: PLEXdb Curator
Name: Nathan,M,Springer
Institution: University of Minnesota, Saint Paul, Minnesota
Head of Laboratory: --
email(s):
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