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Browse MaizePLEX Experiment Data

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Transcriptional and translational gene regulation in the maize pulvinus
Henrietta,,Myburg, NC State University, Raleigh, North Carolina (cassi_myburg@ncsu.edu)
Experiment design (48 hybridizations)
gravity stimulation
•control •2 min •5 min •15 min •30 min •60 min
pulvinus segment
•upper •lower
RNA type
•total •polyribosme-associated

This experiment has been imported by PLEXdb from NCBI GEO (GSE8320)

Series_summary
Not much is known about the molecular processes in...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcriptional and translational gene regulation in the maize pulvinus
Accession No: ZM19
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
gravity stimulation
•control   •2 min   •5 min   •15 min   •30 min   •60 min
pulvinus segment
•upper   •lower
RNA type
•total   •polyribosme-associated
Quality Control: biological replicates
Treatment summary:
 gravity stimulation  pulvinus segment  RNA type  # replicates
 control  upper  total  2
 control  upper  polyribosme-associated  2
 control  lower  total  2
 control  lower  polyribosme-associated  2
 2 min  upper  total  2
 2 min  upper  polyribosme-associated  2
 2 min  lower  total  2
 2 min  lower  polyribosme-associated  2
 5 min  upper  total  2
 5 min  upper  polyribosme-associated  2
 5 min  lower  total  2
 5 min  lower  polyribosme-associated  2
 15 min  upper  total  2
 15 min  upper  polyribosme-associated  2
 15 min  lower  total  2
 15 min  lower  polyribosme-associated  2
 30 min  upper  total  2
 30 min  upper  polyribosme-associated  2
 30 min  lower  total  2
 30 min  lower  polyribosme-associated  2
 60 min  upper  total  2
 60 min  upper  polyribosme-associated  2
 60 min  lower  total  2
 60 min  lower  polyribosme-associated  2
Total hybridizations: 48
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE8320)

Series_summary
Not much is known about the molecular processes involved during gravitropism in monocot plants such as maize. A microarray based study on the expression of genes after a gravity stimulating the maize pulvinus will provide us with valuable information and a better understanding of the underlying molecular processes involved in monocot gravitropism.
Objectives for this study included the identification of genes that were regulated at the transcriptional and translation level during gravitropism in the maize pulvinus. This was achieved by microarray analysis of total RNA versus polyribosome associated RNA during a time-course of gravity stimulation of the maize pulvinus.
Keywords: time course

Series_overall_design:
Six week old maize plants were gravity stimulated by 90º reorientation. Upper (slow elongation) and lower (fast elongation) halves of the most gravity competent pulvini were harvested over a time course ranging from 2 minutes up to one hour (2min, 5 min, 15min, 30min, 60min). Pulvini samples from control (vertical, no gravity stimulation) plants were harvested and labeled as left and right. For each time point, total mRNA and polyribosome-associated mRNA were purified and the transcript profiles analyzed using Affymetrix GeneChip® Maize Genome Arrays. The experiment was repeated twice (two growing seasons) and represent two biological repetitions.

PLEXdb Curator's Note:
1.
To form the factorial structure of this experiment, I have assumed the following: The left/right pulvinus segments of the control plants(vertical) are equivalent to the upper/lower segments of the gravity stimulated plants.

2.
The 'organism part' in all the samples in this experiment is: pulvinus ; PO:0009000
Publication: none
Created: 2009-04-28 13:20:29
Last Update: 2009-04-28 15:07:38
GEO Accession GSE8320
Submitter: PLEXdb Curator
Name: Henrietta,,Myburg
Institution: NC State University, Raleigh, North Carolina
Head of Laboratory: --
email(s):
Homepage:

 
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