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Browse MaizePLEX Experiment Data

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Maize gene expression during infection with Ustilago maydis
Jörg,Thomas,Kämper, Max-Planck-Institute for terrestrial Microbiology, Marburg, Germany (kaemper@mpi-marburg.mpg.de)
Experiment design (36 hybridizations)
pathogen infection
•mock •infected
time
•0.5 dpi •1 dpi •2 dpi •4 dpi •4.5 dpi •8 dpi

This experiment has been imported by PLEXdb from NCBI GEO (GSE10023)

Series_summary:
The fungal pathogen Ustilago maydis establishes ...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Maize gene expression during infection with Ustilago maydis
Accession No: ZM20
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen infection
•mock   •infected
time
•0.5 dpi  •1 dpi  •2 dpi  •4 dpi  •4.5 dpi  •8 dpi
Quality Control: biological replicates
Treatment summary:
 pathogen infection  time  # replicates
 mock  0.5 dpi  3
 mock  1 dpi  3
 mock  2 dpi  3
 mock  4 dpi  3
 mock  4.5 dpi  3
 mock  8 dpi  3
 infected  0.5 dpi  3
 infected  1 dpi  3
 infected  2 dpi  3
 infected  4 dpi  3
 infected  4.5 dpi  3
 infected  8 dpi  3
Total hybridizations: 36
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE10023)

Series_summary:
The fungal pathogen Ustilago maydis establishes a biotrophic relationship with its host plant maize. Hallmarks of the disease are large plant tumors in which fungal proliferation occurs. Plants have developed various defense pathways to cope with pathogens. We used microarrays to detail the global programme of gene expression during the infection process of Ustilago maydis in its host plant to get insights into the defense programs and the metabolic reprogramming needed to supply the fungus with nutrients.

Keywords: time course

Series_overall_design:
In three independent experiments plants were infected with the solopathogenic U. maydis strain SG200. Samples from infected leaves were taken at 12 and 24 hours post infection, as well as 2, 4 and 8 days post infection. Samples from uninfected control plants were taken at the same time points.

PLEXdb Curator's Note:
The following three samples in the source GEO Series have not been in included in this PLEXdb experiment. They could not be unambiguously assigned to any of the treatment factors described in the Series Overall Design.

GSM253280 ZM_8d_SG200_3Alu_I
GSM253281 ZM_8d_SG200_3Alu_II
GSM253282 ZM_8d_SG200_3Alu_III
Publication: none
Created: 2009-04-28 15:15:39
Last Update: 2009-04-28 16:00:46
GEO Accession GSE10023
Submitter: PLEXdb Curator
Name: Jörg,Thomas,Kämper
Institution: Max-Planck-Institute for terrestrial Microbiology, Marburg, Germany
Head of Laboratory: --
email(s):
Homepage:

 
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