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Browse MaizePLEX Experiment Data

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Maize gene expression during infection with Ustilago maydis strain SG200Dpep1
Gunther,,Doehlemann, Max Planck Institue for Terrestiral Microbiology, Marburg, Germany (doehlemann@mpi-marburg.mpg.de)
Experiment design (6 hybridizations)
pathogen infection
•mock •infected

This experiment has been imported by PLEXdb from NCBI GEO (GSE12892)

Series_summary:
The basidiomycete Ustilago maydis causes smut di...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Maize gene expression during infection with Ustilago maydis strain SG200Dpep1
Accession No: ZM24
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen infection
•mock   •infected
Quality Control: biological replicates
Treatment summary:
 pathogen infection  # replicates
 mock  3
 infected  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE12892)

Series_summary:
The basidiomycete Ustilago maydis causes smut disease in maize. Colonization of the host plant is initiated by direct penetration of cuticle and cell wall of maize epidermis cells. The invading hyphae are surrounded by the plant plasma membrane and proliferate within the plant tissue. We identified a novel secreted protein, termed Pep1. Disruption mutants of pep1 are not affected in saprophytic growth and develop normal infection structures. However, Δpep1 mutants fail to penetrate the epidermal cell wall and elicit a strong plant defense response. Using Affymetrix maize arrays we identified about 110 plant genes which are differentially regulated in Δpep1 and wild type infections during the penetration stage.

Series_overall_design:
In three independent experiments plants were infected with the strain SG200Dpep1 which is derived from the solopathogenic U. maydis strain SG200. Samples from infected leaves were taken at 24 hours post infection. Samples were treated under the same conditions as described previously.
Publication: 'Pep1, a secreted effector protein of Ustilago maydis, is required for successful invasion of plant cells.', Doehlemann G, van der Linde K, Assmann D, Schwammbach D et al.
PLoS Pathog 2009 Feb;5(2):e1000290.
pubmed: 19197359
Created: 2009-04-30 13:33:20
Last Update: 2009-04-30 13:43:57
GEO Accession GSE12892
Submitter: PLEXdb Curator
Name: Gunther,,Doehlemann
Institution: Max Planck Institue for Terrestiral Microbiology, Marburg, Germany
Head of Laboratory: --
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