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Genome-wide transcriptome analysis of two maize inbred lines under drought stress during the seedling stage
Guoying Wang, Chinese Academy of Agricultural Sciences, Institute of Crop Sciences, Beijing, China (gywang@caas.net.cn)
Experiment design (24 hybridizations)
genetic line
•Han21(drought-tolerant) •Ye478(drought-sensitive)
stress condition
•control •moderate drought •severe drought •re-watering

This experiment has been imported by PLEXdb from NCBI GEO (GSE16567) Series_summary: To understand the transcriptome changes during drought to...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Genome-wide transcriptome analysis of two maize inbred lines under drought stress during the seedling stage
Accession No: ZM30
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•Han21(drought-tolerant)   •Ye478(drought-sensitive)
stress condition
•control   •moderate drought   •severe drought   •re-watering
Quality Control: biological replicates
Treatment summary:
 genetic line  stress condition  # replicates
 Han21(drought-tolerant)  control  3
 Han21(drought-tolerant)  moderate drought  3
 Han21(drought-tolerant)  severe drought  3
 Han21(drought-tolerant)  re-watering  3
 Ye478(drought-sensitive)  control  3
 Ye478(drought-sensitive)  moderate drought  3
 Ye478(drought-sensitive)  severe drought  3
 Ye478(drought-sensitive)  re-watering  3
Total hybridizations: 24
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE16567)

Series_summary:
To understand the transcriptome changes during drought tolerance in maize, the drought-tolerant line Han21 and drought-sensitive line Ye478, which show substantial differences in drought tolerance at the seedling stage, were selected for this study. Using the GeneChip Maize Genome Arrays, we applied genome-wide gene expression analysis to the two genotypes under gradual drought stress and re-watering. We identified 2172 common regulated transcripts in both lines under drought stress, with 1084 common up-regulated transcripts and 1088 common down-regulated transcripts. Among the 2172 transcripts, 58 potential protein kinases and 117 potential transcription factors were identified. The potential components of the ABA signaling pathway were identified from the common regulated transcripts. We also identified 940 differentially regulated transcripts between the two lines. Among the 940 transcripts, the differential expression levels of 29 transporters and 15 cell wall-related transcripts may contribute to the different tolerances of the two lines. Additionally, we found that the drought-responsive genes in the tolerant Han21 line recovered more quickly when the seedlings were re-watered, and 311 transcripts in the tolerant Han21 line were exclusively up-regulated at the re-watering stage compared to the control and stress conditions. Our study provides a global characterization of two maize inbred lines during drought stress and re-watering and will be valuable for further study of the molecular mechanisms of drought tolerance in maize.

Series_overall_design:
In two independent experiments, we generate maize gene expression profiles during drought stress and re-watering through comparing genome-wide expression patterns of drought stress treatment and re-watering treatment by using 17,555 Affymetrix maize whole genome array.
Publication: 'Genome-wide transcriptome analysis of two maize inbred lines under drought stress.', Zheng J, Fu J, Gou M, Huai J, Liu Y, Jian M, Huang Q, Guo X, Dong Z, Wang H, Wang G.
Plant Mol Biol 2010 Mar;72(4-5):407-21.
pubmed: 19953304
Created: 2011-02-25 11:40:15
Last Update: 2011-02-25 13:09:50
Released: 2011-03-04
GEO Accession GSE16567
Submitter: PLEXdb Curator
Name: Guoying Wang
Institution: Chinese Academy of Agricultural Sciences, Institute of Crop Sciences, Beijing, China
Head of Laboratory: Guoying Wang
email(s):
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