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Expression profile of contrasting maize genotypes grown in acid and control soil (leaves)
Marcelo Menossi, Universidade Estadual de Campinas, Departamento de Genética, Campinas, Brazil (menossi@unicamp.br)
Experiment design (12 hybridizations)
genetic line
•Cat100-6(Al-tolerant) •S1587-17(Al-sensitive)
growth condition
•control soil •acid soil

This experiment has been imported by PLEXdb from NCBI GEO (GSE22479) Series_summary: Aluminum toxicity is one of the major limiting factors for...[complete overview]

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Experiment Name: Expression profile of contrasting maize genotypes grown in acid and control soil (leaves)
Accession No: ZM35
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•Cat100-6(Al-tolerant)   •S1587-17(Al-sensitive)
growth condition
•control soil   •acid soil
Quality Control: biological replicates
Treatment summary:
 genetic line  growth condition  # replicates
 Cat100-6(Al-tolerant)  control soil  3
 Cat100-6(Al-tolerant)  acid soil  3
 S1587-17(Al-sensitive)  control soil  3
 S1587-17(Al-sensitive)  acid soil  3
Total hybridizations: 12
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE22479)

Series_summary:
Aluminum toxicity is one of the major limiting factors for many crops worldwide. The primary symptom of Al toxicity syndrome is the inhibition of root growth, leading to poor water and nutrient absorption. The causes of this inhibition are still elusive, with several biochemical pathways being affected and with a significant variation between species. Most of the work done so far to investigate the genes responsible for Al tolerance used hydroponic culture. Here we evaluated plant responses using soil as substrate, which is a condition closer to the field reality.
We used Affymetrix chips to reveal the transcriptional changes of two maize genotypes contrasting for Al tolerance.

Series_overall_design:
Leaves from Cat100-6 (Al-tolerant) and S1587-17 (Al-sensitive) maize genotypes were harvested after growing on acid or control soil for 3 days. Total RNA was extracted and hybridized to Affymetrix GeneChip Maize Genome Array. Three biological replicates were used for each sample.
Publication: none
Created: 2011-03-04 11:23:28
Last Update: 2011-03-04 11:41:45
Released: 2011-03-10
GEO Accession GSE22479
Submitter: PLEXdb Curator
Name: Marcelo Menossi
Institution: Universidade Estadual de Campinas, Departamento de Genética, Campinas, Brazil
Head of Laboratory: Marcelo Menossi
email(s):
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