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Browse MaizePLEX Experiment Data

Choose an experiment:
affy_ams_maize-Cell wall maize mutant during arbuscular mycorrhizal symbiosis
Stephanie Huguet, INRA, Department of URGV, Evry, France (huguet@evry.inra.fr)
Experiment design (8 hybridizations)
genotype
•F2(WT) •bm4(mut)
pathogen inoculation
•control(tem) •mycorrhiza(myc)

This experiment has been imported by PLEXdb from NCBI GEO (GSE22950)

Series_summary:
affy_ams_maize - aafy_ams_maize - Main objective...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: affy_ams_maize-Cell wall maize mutant during arbuscular mycorrhizal symbiosis
Accession No: ZM42
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•F2(WT)   •bm4(mut)
pathogen inoculation
•control(tem)   •mycorrhiza(myc)
Quality Control: biological replicates
Treatment summary:
 genotype  pathogen inoculation  # replicates
 F2(WT)  control(tem)  2
 F2(WT)  mycorrhiza(myc)  2
 bm4(mut)  control(tem)  2
 bm4(mut)  mycorrhiza(myc)  2
Total hybridizations: 8
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE22950)

Series_summary:
affy_ams_maize - aafy_ams_maize - Main objectives of this study: - role of plant cell wall during AM symbiosis - regulation pattern during AM symbiosis - understanding of the original mycorrhizal phenotype of bm4 - search of candidate-genes to study AM symbiosis-After 7 days in phytochambers, plantlets were transplanted in 2L of humidified bedrock with 3000 spores for inoculation conditions or without spore for mock conditions then plants have grown in greenhouse. Maize roots have been sampled 7 weeks post-inoculation and freezed in liquid nitrogen then stocked at -80°C. Total RNA was isolated from frozen root tissue by using the RNeasy Plant Mini Kit (Qiagen) following the manufacturer’s instructions.
Keywords: treated vs untreated comparison

Series_overall_design:
8 arrays - maize
Publication: none
Created: 2011-08-05 11:23:26
Last Update: 2011-08-10 10:55:28
Released: 2011-08-10
GEO Accession GSE22950
Submitter: PLEXdb Curator
Name: Stephanie Huguet
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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