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Browse MaizePLEX Experiment Data

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Maize gene expression during infection with Ustilago maydis strains SG200Dpit1 and SG200Dpit2
Gunther Doehlemann, Max Planck Institue for Terrestiral Microbiology, Department of Organismic Interactions, Marburg, Germany (doehlemann@mpi-marburg.mpg.de)
Experiment design (6 hybridizations)
pathogen strains
•SG200Dpit1 •SG200Dpit2

This experiment has been imported by PLEXdb from NCBI GEO (GSE28479)

Series_summary:
Ustilago maydis is a basidiomycete fungus that c...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Maize gene expression during infection with Ustilago maydis strains SG200Dpit1 and SG200Dpit2
Accession No: ZM47
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
pathogen strains
•SG200Dpit1   •SG200Dpit2
Quality Control: biological replicates
Treatment summary:
 pathogen strains  # replicates
 SG200Dpit1  3
 SG200Dpit2  3
Total hybridizations: 6
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE28479)

Series_summary:
Ustilago maydis is a basidiomycete fungus that causes smut disease in maize. Most prominent symptoms of the disease are plant tumors, which can be induced by U. maydis on all aerial parts of the plant. We identified two linked genes, pit1 and pit2, which are specifically expressed during plant colonization. Deletion mutants for either pit1 or pit2 are unable to induce tumor development and elicit plant defense responses.
We used the Affymetrix maize genome array to analyze the transcriptional responses of maize to deletion pit1 and pit2 mutants and found plant responses to both mutants being not significantly distinguishable.

Series_overall_design:
U. maydis infected parts of maize seedling leaves were dissected 4 days after inoculation with strain SG200Dpit1 and SG200Dpit2, respectively. We previously submitted data of maize leaves that were treated with the progenitor wild type strain SG200 as well as mock-infections under identical experimetal conditions (GEO: GSE10023, 4d mock and 4d SG200 Samples). These data served as controls for this experiment.
Publication: none
Created: 2011-09-22 10:33:19
Last Update: 2011-09-27 09:39:09
Released: 2011-09-27
GEO Accession GSE28479
Submitter: PLEXdb Curator
Name: Gunther Doehlemann
Institution: Max Planck Institue for Terrestiral Microbiology, Department of Organismic Interactions, Marburg, Germany
Head of Laboratory: Gunther Doehlemann
email(s):
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