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Browse MaizePLEX Experiment Data

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Gene expression change caused by copy number variations
Tao Zuo, Iowa State University (taozuo@iastate.edu)
Experiment design (9 hybridizations)
genotype
•p1-ww714/p1-ww714 •p1-ww714/B73 •B73/B73

We isolated a number of duplications and deletions, and conducted expression analysis on one case (p1-ww714), which contains an inverted duplicatio...[complete overview]

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Experiment Name: Gene expression change caused by copy number variations
Accession No: ZM50
Microarray: Maize100WT_maize
Visibility: public
Experiment Type:
Experiment Factor(s):
genotype
•p1-ww714/p1-ww714   •p1-ww714/B73   •B73/B73
Quality Control: biological replicates,technical replicates
Treatment summary:
 genotype  # replicates
 p1-ww714/p1-ww714  3
 p1-ww714/B73  3
 B73/B73  3
Total hybridizations: 9
Description: We isolated a number of duplications and deletions, and conducted expression analysis on one case (p1-ww714), which contains an inverted duplication on chromosome 1S. The region duplicated in p1-ww714 is 14.7 Mb in size and is predicted to contain approximately 300 gene models according to Maize GDB.

Three genotypes were used in this experiment. One is homozygous of p1-ww714 allele which contains four copies of 14.7 Mb segment. The other two are B73 allele and heterozygous allele of B73/p1-ww714 which contain 2 and 3 copies of this segment respectively.

RNA samples of six plants from each genotype were pooled for hybridization and three replications were conducted for each genotype.
Publication: none
Created: 2012-02-14 11:22:41
Last Update: 2012-02-16 19:00:10
Released: 2015-10-19
Submitter: Thomas Peterson
Name: Tao Zuo
Institution: Iowa State University
Head of Laboratory: Thomas Peterson
email(s):
Homepage:

 
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