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Browse MaizePLEX Experiment Data

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affy_zeawall_ril_maize - Expression studies in 5 early maize RIL with contracted allelic patterns at major QTL posititions
Sandrine Balzergue, INRA, Department of URGV, Evry, France (balzerg@evry.inra.fr)
Experiment design (20 hybridizations)
genetic line
•RIL 99 •RIL 54 •RIL 39 •RIL 71 •RIL 118
developmental stage name
•emerging tassel •early silking

This experiment has been imported by PLEXdb from NCBI GEO (GSE22439)

Series_summary:
affy_zeawall_ril_maize - Four major QTL explainin...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: affy_zeawall_ril_maize - Expression studies in 5 early maize RIL with contracted allelic patterns at major QTL posititions
Accession No: ZM55
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
genetic line
•RIL 99   •RIL 54   •RIL 39   •RIL 71   •RIL 118
developmental stage name
•emerging tassel   •early silking
Quality Control: biological replicates
Treatment summary:
 genetic line  developmental stage name  # replicates
 RIL 99  emerging tassel  2
 RIL 99  early silking  2
 RIL 54  emerging tassel  2
 RIL 54  early silking  2
 RIL 39  emerging tassel  2
 RIL 39  early silking  2
 RIL 71  emerging tassel  2
 RIL 71  early silking  2
 RIL 118  emerging tassel  2
 RIL 118  early silking  2
Total hybridizations: 20
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE22439)

Series_summary:
affy_zeawall_ril_maize - Four major QTL explaining lignin content and cell wall degradability variation have been identified in the F288 x F271 RIL progeny (Roussel et al., 2002, Maydica 47: 9-20). Gene expression has been considered as a way in identification of candidate gene underlying QTL. Five lines chosen for their allelic pattern at four main QTL were considered for expression studies, two lines with all four favorable alleles (RIL99, RIL54), two lines with the unfavorable allele at bin 6.06 QTL position (RIL39 , RIL71), and one line with the unfavorable allele at bin 9.02 QTL position (RIL118). Expression study will be investigated in ear internode at two stages emerging tassel and early silking stages with two replicates. Plants were cropped at INRA Lusignan (Vienne, France) in 2009 in a bloc design with two four-row replicates. Below-ear internodes of five representative RIL plants of one row in each replicate were harvested at the two stages and pooled.
Keywords: genotype comparison, time course

Series_overall_design:
20 arrays - maize
Publication: none
Created: 2012-07-19 10:49:59
Last Update: 2012-07-19 12:36:16
Released: 2012-07-19
GEO Accession GSE22439
Submitter: PLEXdb Curator
Name: Sandrine Balzergue
Institution: INRA, Department of URGV, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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