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Browse MaizePLEX Experiment Data

Choose an experiment:
affy_zeawall_ril2_maize: ZeaWall Project, defavorable parent F271
Stephanie Huguet, INRA, Evry, France (huguet@evry.inra.fr)
Experiment design (4 hybridizations)
developmental stage name
•emerging tassel •early silking

This experiment has been imported by PLEXdb from NCBI GEO (GSE27070)

Series_summary:
affy_zeawall_ril2_maize - affy_zeawall_ril2_maiz...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: affy_zeawall_ril2_maize: ZeaWall Project, defavorable parent F271
Accession No: ZM57
Microarray: maize18k
Visibility: public
Experiment Type:
Experiment Factor(s):
developmental stage name
•emerging tassel   •early silking
Quality Control: biological replicates
Treatment summary:
 developmental stage name  # replicates
 emerging tassel  2
 early silking  2
Total hybridizations: 4
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE27070)

Series_summary:
affy_zeawall_ril2_maize - affy_zeawall_ril2_maize - Four major QTL explaining lignin content and cell wall degradability variation have been identified in the F288 x F271 RIL progeny (Roussel et al., 2002, Maydica 47: 9-20). Gene expression has been considered as a way in identification of candidate gene underlying QTL. Five lines chosen for their allelic pattern at four main QTL were considered for expression studies, two lines with all four favorable alleles (RIL99, RIL54), two lines with the unfavorable allele at bin 6.06 QTL position (RIL39 , RIL71), and one line with the unfavorable allele at bin 9.02 QTL position (RIL118). The defavorable parent F271 was studied in complement and is the object of this last array. Expression studies will be investigated in ear internode at two stages (emerging tassel and early silking).-Five lines chosen for their allelic pattern at four main QTL were considered for expression studies, two lines with all four favorable alleles (RIL99, RIL54), two lines with the unfavorable allele at bin 6.06 QTL position (RIL39 , RIL71), and one line with the unfavorable allele at bin 9.02 QTL position (RIL118). The defavorable parent F271 was also studied in complement and is the object of this last array.Expression study will be investigated in ear internode at two stages emerging tassel and early silking stages with two replicates. Plants were cropped at INRA Lusignan (Vienne, France) in 2009 in a bloc design with two four-row replicates. Below-ear internodes of five representative RIL plants of one row in each replicate were harvested at the two stages and pooled.

Series_overall_design:
4 arrays - maize; time course

PLEXdb Curator Notes:
Key:
pan- emerging tassel
flo- early silking
Publication: none
Created: 2012-10-16 11:06:56
Last Update: 2012-10-16 11:40:49
Released: 2012-10-16
GEO Accession GSE27070
Submitter: PLEXdb Curator
Name: Stephanie Huguet
Institution: INRA, Evry, France
Head of Laboratory: Claire Lurin
email(s):
Homepage:

 
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