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Experiment Name:

Comparative transcriptomic reveals signatures specific to various interactions with Melampsora rust fungi

Accession No:

PT1

Microarray:

Poplar61k

Visibility:

public

Experiment Type:

Experiment Factor(s):

infection: •Non_infected •Mlp_infected •Mmd_infected •Mixed_infected

Quality Control:

biological replicates

Treatment summary:

infection	# replicates
Non_infected	3
Mlp_infected	3
Mmd_infected	3
Mixed_infected	3

Total hybridizations: 12

Description:

This experiment has been imported by PLEXdb from NCBI GEO (GSE9673)

Series_summary:
We aim to understand the global transcriptional response of hybrid poplar NM6 (Populus nigra x Populus maximoviczii) to infection by two biotrophic Melampsora fungi, M. larici-populina and M. medusae f. sp. deltoidae separately and simultanously. Transcript profilling revealed 699 differentially expressed genes whose expression level was equal or higher than 2-fold between infected and noninfected tissues. In the mixed infection, our results showed an additive response of the two pathogens.
Genes regulated as a result of Melampsora infection were generally induced and mainly involved in primary/secondary metabolism that include cell wall reinforcement and lignification, defense-related processes like pathogenesis-related and stress response, signal perception and transduction mechanisms and regulation of transcription.

Keywords: Stress response

Series_overall_design:
Poplar leaves were infected with Melampsora larici-populina and Melampsora medusae f. sp. deltoidae and the mixture of them (Mixed infection). At six day post-infection, tissues were harvested for RNA extraction and hybridization on Affymetrix microarrays. Three biological replications were obtained from infected and non infected leaves (control samples).

Publication:

'Transcriptome profiling in hybrid poplar following interactions with Melampsora rust fungi', Azaiez A, Boyle B, Levée V, Séguin A
Mol Plant Microbe Interact 2009 Feb;22(2):190-200
pubmed: 19132871

Created:

2009-07-06 15:27:28

Last Update:

2009-07-06 15:38:50

GEO Accession

GSE9673

Submitter:

PLEXdb Curator

Experimenter:

Name:	Armand,,Séguin
Institution:	Laurentian forestry Service, Québec, Canada
Head of Laboratory:	--
email:	Armand.Seguin@rncan.gc.ca
Homepage:


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