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Tomato wild type, vector control and transgenic lines expressing InsP 5-ptase: mature fruits, root tips, and leaves
Mariya,,Khodakovskaya, North Carolina State University, Raleigh, NC (mvkhodak@ncsu.edu)
Experiment design (40 hybridizations)
organism part name
•fruits •leaves •root_tips
transgenic
•Wild type •Vector control •Transgen. Line N6 •Transgen. Line N7

This experiment has been imported by PLEXdb from NCBI GEO (GSE9683)

Series_summary: To reduce the level of major second messenger inosi...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Tomato wild type, vector control and transgenic lines expressing InsP 5-ptase: mature fruits, root tips, and leaves
Accession No: LE2
Microarray: tomato10k
Visibility: public
Experiment Type:
Experiment Factor(s):
organism part name
•fruits   •leaves   •root_tips
transgenic
•Wild type   •Vector control   •Transgen. Line N6   •Transgen. Line N7
Quality Control: biological replicates
Treatment summary:
 organism part name  transgenic  # replicates
 fruits  Wild type  4
 fruits  Vector control  4
 fruits  Transgen. Line N6  4
 fruits  Transgen. Line N7  4
 leaves  Wild type  3
 leaves  Vector control  3
 leaves  Transgen. Line N6  3
 leaves  Transgen. Line N7  3
 root_tips  Wild type  3
 root_tips  Vector control  3
 root_tips  Transgen. Line N6  3
 root_tips  Transgen. Line N7  3
Total hybridizations: 40
Description: This experiment has been imported by PLEXdb from NCBI GEO (GSE9683)

Series_summary:
To reduce the level of major second messenger inositol-1,4,5-triphosphate (InsP3), we generated transgenic tomato plants (cv. Micro-Tom) that are expressing InsP 5-ptase gene. To understand effects of transgene (InsP 5-ptase) on gene expression we carried out microarray analysis from different parts of transgenic and control (wild type, empty vector control) tomato plants
Objectives for this study included the identification of genes that were up or down-regulated at the transcriptional level in transgenic tomato plants expressing InsP 5-ptase gene

Keywords: genetic modification

Series_overall_design:
Ripe fruits of wild type, vector control and two transgenic tomato lines expressing InsP 5-ptase gene were selected for RNA extraction and hybridization on Affymetrix microarrays. First two leaves of 10 day old light grown in vitro seedlings of wild type, vector control and two transgenic tomato lines expressing InsP 5-ptase gene were selected for RNA extraction and hybridization on Affymetrix microarrays


10 day old etiolated root tips of wild type, vector control and two transgenic tomato lines expressing InsP 5-ptase gene were selected for RNA extraction and hybridization on Affymetrix microarrays
Publication: none
Created: 2009-07-08 12:48:57
Last Update: 2009-07-08 13:24:09
GEO Accession GSE9683
Submitter: PLEXdb Curator
Name: Mariya,,Khodakovskaya
Institution: North Carolina State University, Raleigh, NC
Head of Laboratory: --
email(s):
Homepage:

 
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