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Browse WheatPLEX Experiment Data

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Transcription patterns during wheat development (Mirrors BB3)
Tim Sutton, Australian Centre for Plant Functional Genomics (tim.sutton@acpfg.com.au)
Experiment design (39 hybridizations)
tissue type
•germinating seed, coleoptile •germinating seed, root •germinating seed, embryo •seedling, root •seedling, crown •seedling, leaf •immature inflorescence •floral bracts, before anthesis •pistil, before anthesis •anthers, before anthesis •3-5 DAP caryopsis •22 DAP embryo •22 DAP endosperm

The analysis of gene expression during wheat development: Gene expression measurements were carried out on a developmental tissue series for wil...[complete overview]

Experiment     Expression     Hybridizations & Samples     Quality Control     Compare Treatments     Downloads    

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Experiment Name: Transcription patterns during wheat development (Mirrors BB3)
Accession No: TA3
Microarray: Wheat
Visibility: public
Experiment Type:
Experiment Factor(s):
tissue type
•germinating seed, coleoptile  •germinating seed, root  •germinating seed, embryo  •seedling, root  •seedling, crown  •seedling, leaf  •immature inflorescence  •floral bracts, before anthesis  •pistil, before anthesis  •anthers, before anthesis  •3-5 DAP caryopsis  •22 DAP embryo  •22 DAP endosperm
Quality Control: biological replicates
Treatment summary:
 tissue type  # replicates
 germinating seed, coleoptile  3
 germinating seed, root  3
 germinating seed, embryo  3
 seedling, root  3
 seedling, crown  3
 seedling, leaf  3
 immature inflorescence  3
 floral bracts, before anthesis  3
 pistil, before anthesis  3
 anthers, before anthesis  3
 3-5 DAP caryopsis  3
 22 DAP embryo  3
 22 DAP endosperm  3
Total hybridizations: 39
Description: The analysis of gene expression during wheat development: Gene expression measurements were carried out on a developmental tissue
series for wild-type wheat (cv. Chinese Spring) using the Affymetrix
Wheat GeneChip. Thirteen tissues at defined developmental stages were
chosen to match the barley (cv. Morex) tissue series of Druka et al.
2006 that used the Affymetrix Barley1 GeneChip. Three replicates of:
root tissue at two different developmental stages, leaf, crown,
caryopsis, anther, pistil, inflorescence, bracts, mesocotyl, endosperm,
embryo and coleoptiles were hybridised. Comparisons between this wheat
data and the barley dataset were performed and are available at
http://contigcomp.acpfg.com.au.

Publication: 'Comparative transcriptomics in the Triticeae', Andreas W Schreiber, Tim Sutton, Rico A Caldo, Elena Kalashyan, Ben Lovell, Gwenda Mayo, Gary J Muehlbauer, Arnis Druka, Robbie Waugh, Roger P Wise, Peter Langridge and Ute Baumann
BMC Genomics 2009, 10:285
pubmed: 19558723
Created: 2006-07-17 22:04:12
Last Update: 2008-08-05 12:18:56
GEO Accession GSE12508
Submitter: Tim Sutton
Name: Tim Sutton
Institution: Australian Centre for Plant Functional Genomics
Head of Laboratory: Peter Langridge
email(s):
Homepage:

 
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